Abstract
Novel Origami Foil Based Microfluidic Foils for DNA Amplification and Multiplex Detection Fabricated by R2R UV NIL
Anne Linhardt, Scientist, JOANNEUM RESEARCH Forschungsgesellschaft mbH
Our pilot line allows patterning of microfluidic channels by R2R UV Nanoimprint Lithography (UV NIL), printing of biomolecules (nucleic acids; aptamers; proteins), by R2R microarray-spotting, and chip closing by R2R lamination. This manufacturing approach with a high degree of process parallelisation (e.g. 50 chips per imprinting tool, throughput of 3600 chip components per hour) leads to a faster and cheaper production compared to the injection moulded chips used so far by the diagnostic platform GENSPEED®. Regarding lab on foil, it is always a challenge to find enough space for liquids (sample, reagents, wash solutions) on the chip, especially if not only multiplex detection but also DNA amplification should fit on the chip size of 7.5 x 2.5 cm2. Therefore a novel origami technique for foil based diagnostic microfluidic chips was developed. After R2R UV NIL and R2R bio-functionalization, the foils with partially cured UV resin were folded and the layers connected by UV light irritation, leading to a chip with three microfluidic channel levels. This three-layer-chip was used to demonstrate on-chip amplification and subsequent detection of multiple analytes.
Anne Linhardt, Scientist, JOANNEUM RESEARCH Forschungsgesellschaft mbH
Dr. Anne Linhardt studied Polymer and Colloid Chemistry, as well as Material Chemistry and Catalysis at the University of Bayreuth. For her doctoral thesis, on biodegradable polyphosphazenes as intravenously administered drug delivery systems, she moved to Johannes Kepler University in Linz in 2013 and completed her PhD studies in polymer chemistry there in 2017. As part of her PhD, she was also able to deepen her interdisciplinary knowledge for three months at the Institute for Bioscience and Biotechnology Research at the University of Maryland. From 2016-2020, she was part of the development team of MEON Medical Solutions GmbH & Co KG in Graz with focus on the development of an analyzer for heterogeneous immunoassays and clinical chemistry. Since mid-2020 she is part of MATERIALS the Institute for Surface Technologies and Photonics of JOANNEUM RESEARCH in Weiz as a research associate. She is mainly engaged in DNA amplification and detection in microfluidics-based systems.
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